Are you centered? An automatic crystal-centering method for high-throughput macromolecular crystallography

Date Published
07/2007
Publication Type
Journal Article
Authors
DOI
10.1107/S0909049507018110
Abstract

Crystal centering is a key step in macromolecular X-ray crystallography experiments. A new method using image-processing and machine-vision techniques allows the centering of small crystals in the X-ray beam. This method positions crystals even when the loop is initially out of the camera's field of view and adapts to the difficulty of the experiment. The process has been tested on many diverse crystals with a 93% success rate when compared with manual centering.

Journal
Journal of synchrotron radiation
Volume
14
Year of Publication
2007
Issue
Pt 4
Pagination
355-60
Keywords
Organizations
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