%0 Journal Article
%K fluorescence
%K separation
%A Matthew N Church
%A Jonathan D Spear
%A Richard E Russo
%A Gregory L Klunder
%A Patrick M Grant
%A Brian D Andresen
%B Analytical Chemistry
%D 1998
%F Laser
%G eng
%N 13
%P 2475-2480
%R 10.1021/ac971043+
%T Transient Isotachophoretic−Electrophoretic Separations of Lanthanides with Indirect Laser-Induced Fluorescence Detection
%V 70
%2 LBNL-41769
%8 07/1998
%! Anal. Chem.
%X <p>Indirect laser-induced fluorescence was used for the detection of several lanthanide species separated by capillary electrophoresis. Quinine sulfate was the fluorescent component of the background electrolyte, and α-hydroxyisobutyric acid was added as a complexing agent to enable the separation of analyte ions that have similar mobilities. The UV lines (333−364 nm) of an argon ion laser were used as the excitation source with a diode array detector for monitoring the fluorescent emission at 442 nm. Electrokinetic injections and transient isotachophoresis were implemented to stack the analyte ions into more concentrated zones. On-line preconcentration factors were determined to be 700 and resulted in limits of detection for La<sup>3+</sup>, Ce<sup>3+</sup>, Pr<sup>3+</sup>, Nd<sup>3+</sup>, Sm<sup>3+</sup>, and Eu<sup>3+</sup> in the low-ppb range (6−11 nM).</p>